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texas red conjugated streptavidin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories texas red conjugated streptavidin
    Texas Red Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red conjugated streptavidin/product/Vector Laboratories
    Average 93 stars, based on 293 article reviews
    texas red conjugated streptavidin - by Bioz Stars, 2026-02
    93/100 stars

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    (A) Western blot analysis to assess biotinylation activity of Split TurboID in tetracycline-induced and rapamycin-added cultures. Cell-free extracts were prepared from HEK-293 TetR+ cells to serve as the negative control. (B) Western blot analysis to demonstrate the efficiency of protein enrichment using <t>streptavidin</t> <t>conjugated</t> magnetic beads. (C) Western blot and SDS-PAGE analyses of the enrichment process. The membrane was probed with HRP-conjugated streptavidin to visualize biotinylated proteins. A replica SDS-PAGE gel was run and stained with silver to visualize proteins. Lane numbers: 1, Wash fraction 1; 2, Wash fraction 2; 3, Elution fraction 1; 4, magnetic beads used in the enrichment process. Nonbiotinylated samples that were also subjected to the enrichment process were from a whole cell-lysate that were prepared from nonbiotinylated cells.
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    Vector Laboratories streptavidin texas red conjugate
    (A) Western blot analysis to assess biotinylation activity of Split TurboID in tetracycline-induced and rapamycin-added cultures. Cell-free extracts were prepared from HEK-293 TetR+ cells to serve as the negative control. (B) Western blot analysis to demonstrate the efficiency of protein enrichment using <t>streptavidin</t> <t>conjugated</t> magnetic beads. (C) Western blot and SDS-PAGE analyses of the enrichment process. The membrane was probed with HRP-conjugated streptavidin to visualize biotinylated proteins. A replica SDS-PAGE gel was run and stained with silver to visualize proteins. Lane numbers: 1, Wash fraction 1; 2, Wash fraction 2; 3, Elution fraction 1; 4, magnetic beads used in the enrichment process. Nonbiotinylated samples that were also subjected to the enrichment process were from a whole cell-lysate that were prepared from nonbiotinylated cells.
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    (A) Western blot analysis to assess biotinylation activity of Split TurboID in tetracycline-induced and rapamycin-added cultures. Cell-free extracts were prepared from HEK-293 TetR+ cells to serve as the negative control. (B) Western blot analysis to demonstrate the efficiency of protein enrichment using <t>streptavidin</t> <t>conjugated</t> magnetic beads. (C) Western blot and SDS-PAGE analyses of the enrichment process. The membrane was probed with HRP-conjugated streptavidin to visualize biotinylated proteins. A replica SDS-PAGE gel was run and stained with silver to visualize proteins. Lane numbers: 1, Wash fraction 1; 2, Wash fraction 2; 3, Elution fraction 1; 4, magnetic beads used in the enrichment process. Nonbiotinylated samples that were also subjected to the enrichment process were from a whole cell-lysate that were prepared from nonbiotinylated cells.
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    (A) Western blot analysis to assess biotinylation activity of Split TurboID in tetracycline-induced and rapamycin-added cultures. Cell-free extracts were prepared from HEK-293 TetR+ cells to serve as the negative control. (B) Western blot analysis to demonstrate the efficiency of protein enrichment using streptavidin conjugated magnetic beads. (C) Western blot and SDS-PAGE analyses of the enrichment process. The membrane was probed with HRP-conjugated streptavidin to visualize biotinylated proteins. A replica SDS-PAGE gel was run and stained with silver to visualize proteins. Lane numbers: 1, Wash fraction 1; 2, Wash fraction 2; 3, Elution fraction 1; 4, magnetic beads used in the enrichment process. Nonbiotinylated samples that were also subjected to the enrichment process were from a whole cell-lysate that were prepared from nonbiotinylated cells.

    Journal: Turkish Journal of Biology

    Article Title: A tetracycline-inducible Split TurboID system for specific biotinylation and identification of nuclear proteins from HEK293T cells

    doi: 10.55730/1300-0152.2734

    Figure Lengend Snippet: (A) Western blot analysis to assess biotinylation activity of Split TurboID in tetracycline-induced and rapamycin-added cultures. Cell-free extracts were prepared from HEK-293 TetR+ cells to serve as the negative control. (B) Western blot analysis to demonstrate the efficiency of protein enrichment using streptavidin conjugated magnetic beads. (C) Western blot and SDS-PAGE analyses of the enrichment process. The membrane was probed with HRP-conjugated streptavidin to visualize biotinylated proteins. A replica SDS-PAGE gel was run and stained with silver to visualize proteins. Lane numbers: 1, Wash fraction 1; 2, Wash fraction 2; 3, Elution fraction 1; 4, magnetic beads used in the enrichment process. Nonbiotinylated samples that were also subjected to the enrichment process were from a whole cell-lysate that were prepared from nonbiotinylated cells.

    Article Snippet: The cells were then incubated with Texas Red conjugated Streptavidin- (Thermo Fisher Sci, USA) for 1 h at room temperature.

    Techniques: Western Blot, Activity Assay, Negative Control, Protein Enrichment, Magnetic Beads, SDS Page, Membrane, Staining